EGFP

基本解釋

EGFP and TK were successfully fused and cloned into pBABE-puro-lox to construct a new vector called pBGTKlox.
成功拼接EGFP-TK融合基因并克隆入pBABE-puro-lox,構(gòu)建成新栽體pBGTKlox。
The percentage of lung metastases in hEndo group, EGFP group and PBS group was 0.0%, 50.0% and 66.7% respectively.
HENDO組、EGFP組、PBS組肺轉(zhuǎn)移率分別為0·0%25、50·0%25、66·7%25;
ALR gene was inserted into pEGFP-Cl plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR.
ALR和EGFP融合基因表達(dá)載體，pEGFP/ALR。
Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells.
將上述基因克隆入綠色熒光蛋白(GFP)表達(dá)載體pIRES2-EGFP中,轉(zhuǎn)染人HeLa細(xì)胞,在熒光顯微鏡和電鏡下觀察轉(zhuǎn)染細(xì)胞的形態(tài)和結(jié)構(gòu)。
Results1. After selection by culturing with G418, clones of transgenic hMSCs were obtained and showed bright EGFP expression.
感染后的hMSCs經(jīng)G418篩選后逐漸形成克隆,克隆中的hMSCs均表達(dá)EGFP,在倒置熒光顯微鏡下可見(jiàn)明亮的綠色熒光。